Extraction of anabolic steroids with aqueous-organic mixtures from hexanic solutions

The extraction of anabolic steroids (1,4-androstadiene-3,17-dione; 17α-methyltestosterone; 19-norethiocholanolone; 4-hydroxytestosterone; 4-androstene-3,17-dione; 5α-androstane-3β,17β-diol; dehydroepiandrosterone; calusterone; clostebol; methandienone; methyldienolone; nandrolone; testosterone; epitestosterone; tibolone; 19-norandrostenedione; 1-methylene-5α-androstan-3α-ol-17-one) by aqueous-organic mixtures from hexane solutions was studied at the temperature (20 ± 1) °C. Based on experimental data obtained, partition ratios were calculated, which used to optimise standard sample preparation procedure in process determining compounds under study biologically active dietary supplements and specialised sports nutrition. It found that most effective selective extractants hydrocarbon are water-acetonitrile containing 10 20 % volume water. An technique has been developed for subsequent gas chromatographic determination them using a triple quadrupole mass spectrometric detector. proposed method is characterised deviation 10–15 detection limit about μg/kg supplements, makes it possible reliably determine impurities prohibited them.